Do you need to characterize your nanomaterials?
We will advise you on which is the appropriate technique for each type of sample to design a specific analysis for each case, depending on the information needed and the type of sample.
Selection of suitable cell lines according to the administration route, biodistribution and expected clearance.
Cell viability assay: MTS in metabolically active cells as indicator of proliferation. Discrimination of viable and non‐viable cells using propidium iodide and trypan blue. xCELLigence system for label-free and real-time monitoring of cell -viability.
Apoptosis: Annexin V / propidium iodide, caspase 3 and caspase 8 activation by flow cytometry Reactive Oxygen Species: determination of reactive oxygen species by flow cytometry.