Do you need to characterize your nanomaterials?
We will advise you on which is the appropriate technique for each type of sample to design a specific analysis for each case, depending on the information needed and the type of sample.
Immunomodulation in PBMCs (peripheral blood mononuclear cells), macrophages and cell lines.
Phagocytosis: Functional evaluation and microscopy monitoring of nanoparticle internalization by phagocytic cells.
Inflammasome: Caspase-1 activation by flow cytometry and induction of IL-1β by enzyme immunoassay (ELISA).
Leukocyte proliferation: Clonal proliferation of PBMCs measured by flow cytometry.
Immunological response: Inflammatory cytokine profile and Th1, Th2, Th17 response of PBMCs measured by flow cytometry or Luminex multiplex technology.
Complement activation: Identification in serum of C3/C3b by western-blot. Quantification of C3b by enzyme immunoassay (ELISA).